In 1824 he co-founded the Glasgow Eye Infirmary and also became the first editor of the Glasgow Medical Journal. His “Treatise on Diseases of the Eye”, published in 1830, was a standard textbook on the subject for over 20 years until the invention of the ophthalmoscope radically changed the practice of ophthalmology. He served as surgeon-oculist to Queen Victoria and would have been President of the Faculty of Physicians and Surgeons, but declined his election to this office on health grounds.

This portrait was presented to the Faculty by Mackenzie's widow in 1884, and his extensive library, including works in several languages on ophthalmology and general surgery, was later donated to the Faculty by his son.
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